| Expression pattern: |
UP |
| Associated gene: |
RUNX3, PVT1, BTRC, IKBA, AGO2 |
| Associated microRNA: |
miR-423-5p |
| Biological function: |
Promotes ESCC cell proliferation, cell cycle progression, and tumor growth; knockdown suppresses proliferation and tumor growth and promotes G2/M cell cycle arrest. |
| Molecular mechanism: |
RUNX3 transcriptionally activates PVT1 to up-regulate circ_0001821; circ_0001821 acts as a ceRNA by sponging miR-423-5p to increase BTRC expression, promoting IKBA ubiquitination and activating NF-kappaB signaling. |
| Biological pathway or process: |
NF-kappaB (promotes); proliferation (promotes); cell cycle (promotes); ceRNA regulation (promotes); ubiquitination (promotes) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Actinomycin D / DRB Stability Assay; RNase R Treatment; RT-qPCR; ISH (In Situ Hybridization); IHC (Immunohistochemistry); RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; ChIP / ChIP-seq; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Cell Cycle Assay; In Vivo Animal Model; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Potential biomarker for ESCC early detection or screening, but no convincing clinical sample validation was performed in this study. |
| Description: |
circ_0001821 is up-regulated in ESCC cells and acts as an oncogenic circRNA. RUNX3 transcriptionally enhances PVT1/circ_0001821 expression, and circ_0001821 promotes ESCC proliferation, cell cycle progression, and tumor growth through the miR-423-5p/BTRC axis, increasing IKBA ubiquitination and activating NF-kappaB signaling. |
| Confidence score: |
0.8363 |