| Expression pattern: |
DN |
| Associated gene: |
ACSL4, AGO2, DHX9 |
| Associated microRNA: |
miR-4291 |
| Biological function: |
inhibits cervical cancer cell proliferation, growth, invasion, tumor growth and metastasis; promotes ferroptosis (and apoptosis-related cell death) |
| Molecular mechanism: |
ceRNA mechanism: circLMO1 sponges miR-4291 to up-regulate ACSL4, thereby facilitating ferroptosis |
| Biological pathway or process: |
ferroptosis (promotes); proliferation (inhibits); invasion (inhibits); metastasis (inhibits); ceRNA regulation (other); apoptosis (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; In Vivo Animal Model; H&E Staining; Bioinformatics Analysis |
| Clinical significance: |
circLMO1 level was associated with FIGO staging; could be a promising biomarker for clinical management of cervical cancer |
| Description: |
circLMO1 (Hsa_circ_0021087) is down-regulated in cervical cancer and suppresses tumor growth, invasion, and metastasis. It mainly localizes to the cytoplasm and functions as a ceRNA by sponging miR-4291, thereby up-regulating ACSL4 and promoting ferroptosis. |
| Confidence score: |
0.849 |