| Expression pattern: |
UP |
| Associated gene: |
c-Myc, SPOP, TXNIP |
| Associated microRNA: |
- |
| Biological function: |
promotes proliferation; promotes migration; promotes metastasis; promotes glucose metabolism (Warburg effect); increases tumor growth in vivo |
| Molecular mechanism: |
CircECE1 binds c-Myc and prevents SPOP-mediated ubiquitination and degradation of c-Myc, leading to c-Myc-dependent repression of TXNIP transcription and activation of the Warburg effect. |
| Biological pathway or process: |
glycolysis (promotes); proliferation (promotes); migration (promotes); metastasis (promotes); Warburg effect (promotes); ubiquitination (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; RT-qPCR; RNase R Treatment; divergent primers PCR; Sanger Sequencing; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Co-IP; RNA-seq; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Luciferase Reporter Assay; ChIP / ChIP-seq; Western Blot; IHC (Immunohistochemistry); IF (Immunofluorescence); In Vivo Animal Model; H&E Staining |
| Clinical significance: |
CircECE1 was higher in osteosarcoma tissues than in chondroma tissues and higher in pulmonary metastases than in primary OS tissues. |
| Description: |
CircECE1 (hsa_circ_0002402), derived from ECE1, is up-regulated in osteosarcoma tissues/cells and promotes proliferation, migration, metastasis, and enhanced glucose metabolism (Warburg effect). It binds c-Myc to block SPOP-mediated ubiquitination and degradation of c-Myc, thereby activating the c-Myc/TXNIP axis to drive metabolic reprogramming in OS. |
| Confidence score: |
0.8776 |