| Expression pattern: |
DN |
| Associated gene: |
PTEN, PI3K/AKT pathway, Ago2 |
| Associated microRNA: |
miR-1323 |
| Biological function: |
circLARP4 suppresses ESCC cell proliferation, migration and EMT, promotes apoptosis, and hampers ESCC tumorigenesis. |
| Molecular mechanism: |
circLARP4 acts as a ceRNA sponge for miR-1323, positively regulates PTEN, and inhibits the PI3K/AKT pathway in ESCC. |
| Biological pathway or process: |
PI3K/AKT (inhibits); proliferation (inhibits); migration (inhibits); apoptosis (promotes); EMT (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Actinomycin D / DRB Stability Assay; RNase R Treatment; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; EdU Staining; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Bioinformatics Analysis |
| Clinical significance: |
circLARP4 may offer a novel and efficient agent for improvement in ESCC prognosis and treatment. |
| Description: |
circLARP4 is down-regulated in ESCC cell lines and acts as a tumor-suppressive circRNA. It inhibits proliferation, migration, EMT and PI3K/AKT signaling while promoting apoptosis, primarily by sponging miR-1323 and relieving repression of PTEN. |
| Confidence score: |
0.7922 |