| Expression pattern: |
DN |
| Associated gene: |
GNAS, Ago2 |
| Associated microRNA: |
miR-142-3p |
| Biological function: |
Circ-BNC2 suppresses OSCC cell proliferation, migration, invasion and tumor growth, induces apoptosis, G0/G1 cell cycle arrest and oxidative stress, and acts as a tumor suppressor in OSCC. |
| Molecular mechanism: |
Circ-BNC2 functions through a ceRNA-like mechanism by binding miR-142-3p, thereby relieving miR-142-3p-mediated repression of GNAS and upregulating GNAS expression. |
| Biological pathway or process: |
ceRNA regulation (other); proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes); cell cycle (inhibits); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Clinical Sample Validation; Transfection; MTT; Colony Formation Assay; Cell Cycle Assay; Transwell Assay; Western Blot; IHC (Immunohistochemistry); Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
Circ_0086414/circ-BNC2 has diagnostic value in distinguishing OSCC from healthy controls, is associated with tumor size and lymphatic metastasis, and may be a promising therapeutic target for OSCC. |
| Description: |
Circ-BNC2 is downregulated in OSCC tissues and cells and acts as a tumor-suppressive circRNA. Its overexpression inhibits OSCC proliferation, migration, invasion and xenograft tumor growth while promoting apoptosis, cell cycle arrest and oxidative stress. Mechanistically, circ-BNC2 binds miR-142-3p to upregulate GNAS expression through the circ-BNC2/miR-142-3p/GNAS axis. |
| Confidence score: |
0.8567 |