circRNA basic information
circBase ID: hsa_circ_0007746
Name: hsa_circ_RPN2
Synonym: circRPN2
Host Gene: RPN2
Genomic location(hg19): chr20:35835675-35838568:+
Genomic location(hg38): chr20:37207272-37210165:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

primary HCC tissues; snap-frozen tumor tissues; paraffin-embedded tissue samples; primary tumor tissues; HCC tumors; paratumors; normal tissues
Cell lines:

L0-2; MHCC97L; MHCC97H; HCCLM3; HepG2; PLC/PRF/5; 293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: ENO1, FOXO1, AKT, mTOR
Associated microRNA: miR-183-5p
Biological function: Suppresses HCC aerobic glycolysis, progression, proliferation, colony formation, migration, invasion, and metastasis; enhances oxidative respiration; acts as a tumor suppressor and prognostic biomarker.
Molecular mechanism: CircRPN2 binds ENO1 and accelerates ubiquitin/proteasome-dependent ENO1 degradation, thereby inhibiting ENO1-mediated AKT/mTOR signaling and aerobic glycolysis; it also functions as a ceRNA sponge for miR-183-5p to increase FOXO1 expression.
Biological pathway or process:

glycolysis (inhibits); PI3K/AKT/mTOR (inhibits); proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); ceRNA regulation (other); ubiquitination (promotes); oxidative phosphorylation (promotes)
Detected method:
Q
H
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; circRNA-seq; RT-qPCR; RNA-seq; FISH / smFISH; ISH (In Situ Hybridization); IHC (Immunohistochemistry); Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Actinomycin D / DRB Stability Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis
Clinical significance:

Low circRPN2 expression is associated with shorter overall survival and higher recurrence; circRPN2 expression, alone or with ENO1 and FOXO1, is an independent prognostic indicator for HCC.
Description:

CircRPN2 is a human HCC-associated circular RNA that is downregulated in metastatic or recurrent HCC and in highly metastatic HCC cell lines. It suppresses aerobic glycolysis, proliferation, migration, invasion, progression, and metastasis by binding ENO1 to promote ubiquitin/proteasome-dependent degradation and by sponging miR-183-5p to increase FOXO1 expression. Clinically, higher circRPN2 expression is associated with better survival and lower recurrence, supporting its role as a prognostic biomarker and potential therapeutic target.
Confidence score:

0.9003
Other information
Title:

CircRPN2 Inhibits Aerobic Glycolysis and Metastasis in Hepatocellular Carcinoma.

Journal: Cancer research
Published: 2022
PubMed ID: 35045986
Study type:

combined biological and clinical study
Data availability: Supplementary data for this article are available at Cancer Research Online (http://cancerres.aacrjournals.org/).
Code availability: -