| Expression pattern: |
UP |
| Associated gene: |
hTERT, AGO2, FMN2 |
| Associated microRNA: |
miR-1182 |
| Biological function: |
Promotes CRC cell proliferation, migration, tumor growth, cell cycle progression and tumorigenesis; knockdown inhibits proliferation and migration in vitro and tumor growth in vivo. |
| Molecular mechanism: |
circFMN2 acts as a ceRNA sponge for miR-1182 and up-regulates hTERT expression through the circFMN2/miR-1182/hTERT axis. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); metastasis (promotes); cell cycle (promotes); apoptosis (other); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Microarray; Clinical Sample Validation; Bioinformatics Analysis; circRIP; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model |
| Clinical significance: |
High circFMN2 expression is associated with larger tumor size, advanced tumor stage, distant metastasis and TNM stage; serum exosomal circFMN2 may be a novel clinical molecular marker, biomarker and therapeutic target for CRC. |
| Description: |
circFMN2 is an up-regulated oncogenic circRNA in CRC tissues, cell lines and serum exosomes. It promotes CRC proliferation, cell cycle progression, migration and xenograft tumor growth mainly by sponging miR-1182 and increasing hTERT expression, and it may serve as a biomarker and therapeutic target. |
| Confidence score: |
0.8571 |