circRNA basic information
circBase ID: -
Name: hsa_circ_EPSTI1
Synonym: circEPSTI1
Host Gene: EPSTI1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0009807
MONDO name: osteosarcoma
Disease details: osteosarcoma / OS
Disease DO ID:
3347
Disease MeSH ID:
D012516
Disease NCIt ID:
C9145
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

adjacent normal tissues; osteosarcoma tissues; lung

Cell lines:

U2OS; MG63

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: MCL1
Associated microRNA: miR-892b
Biological function: circEPSTI1 promotes osteosarcoma cell proliferation, migration, invasion, tumor growth and lung metastasis; knockdown of circEPSTI1 suppresses these malignant phenotypes.
Molecular mechanism: circEPSTI1 acts as a ceRNA and miRNA sponge for miR-892b to regulate MCL1 expression.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes)

Detected method:
Q
Validation methods:

RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; Bioinformatics Analysis

Clinical significance:

circEPSTI1 may serve as a target and biomarker for osteosarcoma treatment.

Description:

circEPSTI1 is upregulated in osteosarcoma and promotes malignant progression, including proliferation, migration, invasion, tumor growth and lung metastasis. Mechanistically, circEPSTI1 functions as a ceRNA by sponging miR-892b to regulate MCL1 expression, suggesting potential value as a therapeutic target and biomarker.

Confidence score:

0.7302

Other information
Title:

circEPSTI1 Acts as a ceRNA to Regulate the Progression of Osteosarcoma.

Journal: Current cancer drug targets
Published: 2020
PubMed ID: 31702512
Study type:

combined biological and clinical study

Data availability: The data supporting the findings of the article is available from the corresponding author on reasonable request; Supplementary material is available on the publisher's web site along with the published article.
Code availability: -