| Expression pattern: |
UP |
| Associated gene: |
BDNF, Ago2 |
| Associated microRNA: |
hsa-miR-107 |
| Biological function: |
Promotes GC cell proliferation and migration; promotes tumorigenesis in vivo. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-107 to upregulate BDNF expression (via Ago2/RISC association). |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; divergent primers PCR; RNase R Treatment; Sanger Sequencing; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Colony Formation Assay; EdU Staining; Transwell Assay; RIP (RNA Immunoprecipitation); Western Blot; In Vivo Animal Model; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
circHIPK3 levels were associated with Tumor & Lymph Node & Metastasis(TNM) stage; ROC AUC=0.743 indicating diagnostic value; described as a potential biomarker/therapeutic target. |
| Description: |
circHIPK3 is upregulated in gastric cancer tissues and cell lines and predominantly localizes to the cytoplasm. It promotes GC cell proliferation and migration and enhances tumor growth in xenografts, mainly by sponging miR-107 to increase BDNF expression (circHIPK3/miR-107/BDNF axis). |
| Confidence score: |
0.8429 |