circRNA basic information
circBase ID: hsa_circ_0000594
Name: hsa_circ_TTBK2
Synonym: -
Host Gene: TTBK2
Genomic location(hg19): chr15:43120125-43164956:-
Genomic location(hg38): chr15:42827927-42872758:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0018666
MONDO name: hepatoblastoma
Disease details: hepatoblastoma / HB
Disease DO ID:
687
Disease MeSH ID:
D018197
Disease NCIt ID:
C3728
Disease ICD11 ID:
1241693063; 1556608523
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

HB tissues; matched non-tumor liver tissues; matched normal liver tissues

Cell lines:

HepG2; Huh6

In vivo animal model:

-

circRNA-disease information
Expression pattern:
UP
Associated gene: SIRT1
Associated microRNA: miR-217
Biological function: hsa_circ_0000594 promotes HB cell proliferation, viability, migration/invasion and malignant phenotypes, and reduces apoptosis; knockdown inhibits malignant phenotype.
Molecular mechanism: hsa_circ_0000594 acts as a miR-217 sponge and regulates the miR-217 target gene SIRT1, forming the hsa_circ_0000594/miR-217/SIRT1 regulatory axis in HB.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (other); metastasis (promotes)

Detected method:
Q
H
Validation methods:

ISH (In Situ Hybridization); RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Luciferase Reporter Assay; Bioinformatics Analysis; Western Blot

Clinical significance:

hsa_circ_0000594 may serve as a novel diagnostic marker, prognostic biomarker, and potential therapeutic target for HB; its expression is associated with the subtype of HB.

Description:

hsa_circ_0000594 is upregulated in hepatoblastoma tissues and may serve as a diagnostic/prognostic biomarker and therapeutic target. Functionally, it supports HB malignant phenotypes by promoting proliferation, viability, migration/invasion and reducing apoptosis. Mechanistically, it acts through a hsa_circ_0000594/miR-217/SIRT1 regulatory axis.

Confidence score:

0.6829

Other information
Title:

Characterization of hsa_circ_0000594 as a new biomarker and therapeutic target for hepatoblastoma.

Journal: European review for medical and pharmacological sciences
Published: 2019
PubMed ID: 31646606
Study type:

combined biological and clinical study

Data availability: CircInteractome; starBase; CircBank; TargetScan; miRDB; miRPathDB; DAVID; STRING
Code availability: -