| Expression pattern: |
UP |
| Associated gene: |
FoxM1, AGO2 |
| Associated microRNA: |
miR-320a |
| Biological function: |
promotes cell proliferation, EMT, migration, invasion, growth and metastasis of cervical cancer |
| Molecular mechanism: |
acts as a ceRNA/miRNA sponge: circCLK3 directly binds and sponges miR-320a, relieving suppression of FoxM1 and promoting FoxM1 expression |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Western Blot; In Vivo Animal Model; H&E Staining; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circCLK3 expression correlates with poor tumor differentiation, advanced FIGO stage, depth of stromal invasion, and predicts shorter overall survival and disease-free survival. |
| Description: |
circCLK3 is up-regulated in cervical cancer and functions as an oncogenic circRNA. It mainly localizes to the cytoplasm and promotes proliferation, EMT, migration, invasion and in vivo growth/metastasis by sponging miR-320a to increase FoxM1 expression (circCLK3/miR-320a/FoxM1 axis). High circCLK3 expression is associated with aggressive clinicopathological features and poorer survival. |
| Confidence score: |
0.8812 |