circRNA basic information
circBase ID: -
Name: hsa_circ_LRP6
Synonym: circLRP6 / CircRNA LRP6
Host Gene: LRP6
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: osteosarcoma / OS
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

OS tissues; paracancerous normal bone tissues
Cell lines:

Saos2; HOS; U2OS; MG63; hFOB1.19
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: LSD1, EZH2, KLF2, APC
Associated microRNA: -
Biological function: CircLRP6 promotes osteosarcoma cell proliferation, migration and invasion, inhibits apoptosis, promotes cell cycle progression, and acts as an oncogene in OS.
Molecular mechanism: CircLRP6 binds LSD1 and EZH2 and mediates their binding to promoter regions of KLF2 and APC, thereby inhibiting KLF2 and APC expression at the transcriptional level.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes); Wnt/beta-catenin (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); ChIP / ChIP-seq; Transfection; CCK8; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Western Blot; Cohort Study; Survival Analysis; ROC Analysis
Clinical significance:

CircLRP6 has diagnostic potential for OS, and high circLRP6 expression is associated with shorter disease-free survival and overall survival; circLRP6 expression is an independent risk factor for OS prognosis.
Description:

CircLRP6 is up-regulated in osteosarcoma tissues and cell lines and high expression predicts poor survival. Functionally, circLRP6 promotes OS cell proliferation, migration, invasion and cell cycle progression while inhibiting apoptosis. Mechanistically, it binds LSD1 and EZH2 to suppress tumor-suppressor genes KLF2 and APC, contributing to OS development.
Confidence score:

0.7923
Other information
Title:

CircRNA LRP6 promotes the development of osteosarcoma via negatively regulating KLF2 and APC levels.

Journal: American journal of translational research
Published: 2019
PubMed ID: 31396323
Study type:

combined biological and clinical study
Data availability: -
Code availability: -