circRNA basic information
circBase ID: hsa_circ_0000418
Name: hsa_circ_CCT2
Synonym: Circ-CCT2
Host Gene: CCT2
Genomic location(hg19): chr12:69983264-69985939:+
Genomic location(hg38): chr12:69589484-69592159:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0018666
MONDO name: hepatoblastoma
Disease details: hepatoblastoma / HB
Disease DO ID:
687
Disease MeSH ID:
D018197
Disease NCIt ID:
C3728
Disease ICD11 ID:
1241693063; 1556608523
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

hepatoblastoma tissues; adjacent normal tissues; subcutaneous tumors

Cell lines:

HuH-6; HepG2; L-02; MIHA; HEK293T

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: TAF15, PTBP1 mRNA
Associated microRNA: -
Biological function: Promotes hepatoblastoma progression (proliferation, migration, invasion, EMT) and inhibits apoptosis; accelerates tumor growth in vivo.
Molecular mechanism: circ-CCT2 binds/recruits TAF15 in the cytoplasm, up-regulates TAF15 protein, and stabilizes PTBP1 mRNA, leading to activation of Wnt/beta-catenin signaling.
Biological pathway or process:

Wnt/beta-catenin (promotes); proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); apoptosis (inhibits)

Detected method:
Q
H
Validation methods:

RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model

Clinical significance:

High circ-CCT2 expression is associated with metastasis in hepatoblastoma patients; proposed as a potential therapeutic target.

Description:

circ-CCT2 is up-regulated in hepatoblastoma and mainly localizes to the cytoplasm. It promotes malignant phenotypes and xenograft tumor growth by recruiting/up-regulating the RBP TAF15, thereby stabilizing PTBP1 mRNA and activating Wnt/beta-catenin signaling.

Confidence score:

0.8232

Other information
Title:

Circ-CCT2 Activates Wnt/beta-catenin Signaling to Facilitate Hepatoblastoma Development by Stabilizing PTBP1 mRNA.

Journal: Cellular and molecular gastroenterology and hepatology
Published: 2024
PubMed ID: 37866478
Study type:

combined biological and clinical study

Data availability: -
Code availability: -