| Expression pattern: |
UP |
| Associated gene: |
PDL1, PD1, AGO2 |
| Associated microRNA: |
miR-34a, miR-28-5p |
| Biological function: |
Promotes PDL1 expression in cancer cells and promotes PD1 expression in CD8+ T cells (via exosomal transfer), inducing T-cell exhaustion and contributing to tumor immune escape. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge: circRNA-002178 sponges miR-34a to relieve repression of PDL1 in tumor cells; exosome-delivered circRNA-002178 sponges miR-28-5p to relieve repression of PD1 in CD8+ T cells; involves AGO2-RISC binding. |
| Biological pathway or process: |
immune regulation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; Western Blot; Flow Cytometry（Non-apoptosis/cycle）; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Serum/plasma exosomal circRNA-002178 is increased in LUAD patients and may serve as an early diagnostic biomarker (high AUC in ROC analysis). |
| Description: |
circRNA-002178 is up-regulated in LUAD tissues/cell lines and is enriched in serum exosomes. It functions as a ceRNA: sponging miR-34a to increase PDL1 in tumor cells and, after exosomal transfer into CD8+ T cells, sponging miR-28-5p to increase PD1, promoting T-cell exhaustion and immune escape; it also shows strong diagnostic potential via exosomal detection. |
| Confidence score: |
0.8684 |