| Expression pattern: |
UP |
| Associated gene: |
PKM2 |
| Associated microRNA: |
miR-122 |
| Biological function: |
promotes glycolysis and induces oxaliplatin chemoresistance in colorectal cancer; exosome-mediated transfer increases resistance in recipient sensitive cells; ciRS-122 knockdown reverses resistance |
| Molecular mechanism: |
Exosomal ciRS-122 acts as a miR-122 sponge, relieving repression on PKM2 and increasing PKM2 protein to enhance glycolysis and chemoresistance (ceRNA-like mechanism). |
| Biological pathway or process: |
glycolysis (promotes); chemoresistance (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; Bioinformatics Analysis; RT-qPCR; Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; Clinical Sample Validation |
| Clinical significance: |
ciRS-122 in serum exosomes is positively correlated with oxaliplatin chemoresistance (higher in PD than PR). |
| Description: |
In colorectal cancer, exosome-delivered hsa_circ_0005963/ciRS-122 is upregulated in oxaliplatin-resistant settings and promotes glycolysis and chemoresistance. Mechanistically, ciRS-122 sponges miR-122 to increase PKM2 (mainly at the protein level), and exosomal si-ciRS-122 can reduce glycolysis and restore oxaliplatin sensitivity in vitro and in vivo. |
| Confidence score: |
0.7283 |