| Expression pattern: |
UP |
| Associated gene: |
- |
| Associated microRNA: |
miR-145 |
| Biological function: |
circPVT1 acts as an oncogene and promotes CRC cell metastasis, migration and invasion; knockdown suppresses migration and invasion. |
| Molecular mechanism: |
circPVT1 functions as a competing endogenous RNA sponge for miR-145, and its metastasis-promoting role is partially achieved through miR-145 sponging. |
| Biological pathway or process: |
metastasis (promotes); migration (promotes); invasion (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; FISH / smFISH; Clinical Sample Validation; Transfection; Transwell Assay; Luciferase Reporter Assay; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
upregulated circPVT1 was closely correlated with poor prognosis and bad clinicopathological features of patients with CRC; high circPVT1 was correlated with advanced clinical stage, metastasis classification, nodal classification, liver metastasis and shorter survival rate. |
| Description: |
In this study, circPVT1 was upregulated in colorectal cancer tissues and cell lines and high expression was associated with poor clinicopathological features and shorter survival. Functionally, circPVT1 promoted CRC cell migration, invasion and metastasis. Mechanistically, cytoplasmic circPVT1 acted as a miR-145 sponge, and the circPVT1/miR-145 axis was proposed as a potential therapeutic target in CRC. |
| Confidence score: |
0.7586 |