| Expression pattern: |
UP |
| Associated gene: |
HMGB1, Ago2, beta-catenin, Wnt3a, c-Myc |
| Associated microRNA: |
miR-665 |
| Biological function: |
promotes proliferation, colony formation, migration, invasion, EMT, and tumor growth/progression in gastric cancer |
| Molecular mechanism: |
ceRNA mechanism: hsa_circ_0006646 sponges miR-665 to upregulate HMGB1 and activate Wnt/beta-catenin signaling, promoting EMT and malignant behaviors |
| Biological pathway or process: |
Wnt/beta-catenin (promotes); proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; Bioinformatics Analysis; RT-qPCR; Clinical Sample Validation; Survival Analysis; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; FISH / smFISH; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model |
| Clinical significance: |
High expression of hsa_circ_0006646 was positively correlated with TNM stage, lymph node invasion and poor prognosis; patients with high levels of hsa_circ_0006646 had significantly lower overall survival rate. |
| Description: |
hsa_circ_0006646 (from PTK2) is up-regulated in gastric cancer and promotes tumor cell proliferation, migration, invasion and EMT. Mechanistically, it functions as a ceRNA that sponges miR-665 to increase HMGB1, thereby activating Wnt/beta-catenin signaling. High hsa_circ_0006646 expression is associated with advanced clinicopathologic features and poorer overall survival. |
| Confidence score: |
0.8768 |