circRNA basic information
circBase ID: hsa_circ_0000519
Name: hsa_circ_RPPH1
Synonym: hsa_cir-cRPPH1_010 / circ_0000519
Host Gene: RPPH1
Genomic location(hg19): chr14:20811436-20811534:-
Genomic location(hg38): chr14:20343277-20343375:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

hepatocellular tumor tissues; adjacent non-tumorous tissue samples; mouse xenograft tumor tissues; pulmonary tissues
Cell lines:

SNU-387; Hep3B; Huh7; MHCC97L; LO2; HUVECs
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: E2F7, Ago2, FGF2, Ang1, VEGF, cyclin D1, cyclin E1
Associated microRNA: miR-1296
Biological function: Promotes HCC cell viability, proliferation, cell cycle progression, angiogenesis, migration, invasion, tumor growth, pulmonary metastasis, and poor prognosis.
Molecular mechanism: hsa_circ_0000519 acts as a miR-1296 sponge and positively regulates E2F7, thereby promoting HCC progression and angiogenesis.
Biological pathway or process:

ceRNA regulation (other); proliferation (promotes); cell cycle (promotes); angiogenesis (promotes); migration (promotes); invasion (promotes); metastasis (promotes)
Detected method:
Q
H
M
Validation methods:

RNase R Treatment; RT-qPCR; Microarray; FISH / smFISH; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Cell Cycle Assay; Transwell Assay; Wound Healing Assay; Tube Formation Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis
Clinical significance:

High hsa_circ_0000519 expression correlated with poor prognosis, advanced T classification, lymph node metastasis, and tumor differentiation in HCC patients; it was proposed as a potential therapeutic target for HCC treatment.
Description:

hsa_circ_0000519 is up-regulated in HCC tissues and cell lines and its high expression is associated with poor prognosis and adverse clinicopathological features. Functionally, it promotes HCC cell proliferation, cell cycle progression, migration, invasion, angiogenesis, tumor growth, and metastasis. Mechanistically, hsa_circ_0000519 sponges miR-1296 to up-regulate E2F7, forming a hsa_circ_0000519/miR-1296/E2F7 axis with potential therapeutic relevance.
Confidence score:

0.8498
Other information
Title:

Circular RNA hsa_circ_0000519 contributes to angiogenesis and tumor progression in hepatocellular carcinoma through the miR-1296/E2F7 axis.

Journal: Human cell
Published: 2023
PubMed ID: 36627545
Study type:

combined biological and clinical study
Data availability: GSE97332; published article; supplementary material available at https:// doi. org/ 10. 1007/ s13577-022-00854-7
Code availability: -