circRNA basic information
circBase ID: hsa_circ_0000586
Name: hsa_circ_UBE3A
Synonym: circUBE3A(2,3,4,5)
Host Gene: UBE3A
Genomic location(hg19): chr15:25650607-25657118:-
Genomic location(hg38): chr15:25405460-25411971:-
Subcellular localization: nucleus and cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008315
MONDO name: prostate cancer
Disease details: prostate cancer
Disease DO ID:
 10283
Disease MeSH ID:
 D011471
Disease NCIt ID:
 C7378
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

PCa tissues; ANP tissues; prostate tissues; lung specimens
Cell lines:

PC-3; DU145; RWPE-1; HEK293T; HLECs; HUVECs
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: adenylate-uridylate-rich binding factor 1 (AUF1), MTHFD2 mRNA, MTHFD2, vimentin, UBE3A
Associated microRNA: -
Biological function: Suppresses prostate cancer cell migration, invasion, metastasis, proliferation, and epithelial-mesenchymal transition.
Molecular mechanism: circUBE3A(2,3,4,5) binds AUF1 and promotes AUF1 nuclear translocation, reducing cytoplasmic AUF1, destabilizing MTHFD2 mRNA, decreasing MTHFD2 protein and vimentin expression, thereby suppressing EMT and metastasis.
Biological pathway or process:

proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); EMT (inhibits); mRNA stability (inhibits)
Detected method:
Q
H
S
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; circRNA-seq; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); IF (Immunofluorescence); Western Blot; Bioinformatics Analysis
Clinical significance:

Downregulated circUBE3A(2,3,4,5) in PCa tissues correlated with higher Gleason score, increased bone metastasis, and higher D'Amico risk classification; it was proposed as a potential diagnostic biomarker and therapeutic target.
Description:

circUBE3A(2,3,4,5) is a human UBE3A-derived circRNA downregulated in prostate cancer tissues and associated with more aggressive clinicopathological features. It acts as a tumor suppressor by binding AUF1, promoting AUF1 nuclear translocation, destabilizing MTHFD2 mRNA, lowering MTHFD2 and vimentin expression, and thereby inhibiting proliferation, migration, invasion, EMT, and metastasis.
Confidence score:

0.8729
Other information
Title:

CircUBE3A(2,3,4,5) promotes adenylate-uridylate-rich binding factor 1 nuclear translocation to suppress prostate cancer metastasis.

Journal: Cancer letters
Published: 2024
PubMed ID: 38423246
Study type:

combined biological and clinical study
Data availability: https://www.mdpi.com/1422-0067/21/21/7812/s1; Supplementary data: https://doi.org/10.1016/j.canlet.2024.216743; available from the corresponding author upon request
Code availability: -