| Expression pattern: |
UP |
| Associated gene: |
KIF2C |
| Associated microRNA: |
miR-548 |
| Biological function: |
promotes BC cell proliferation, migration, invasion and metastasis; promotes tumor growth |
| Molecular mechanism: |
ceRNA mechanism: circRGNEF sponges miR-548 to enhance KIF2C expression |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RNase R Treatment; RT-qPCR; FISH / smFISH; Luciferase Reporter Assay; Transfection; EdU Staining; CCK8; Colony Formation Assay; Transwell Assay; Cell Cycle Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circRGNEF expression was correlated with aggressive BC phenotypes and poorer overall survival. |
| Description: |
circRGNEF (hsa_circ_0072995) is up-regulated in bladder cancer tissues and cell lines and is mainly localized in the cytoplasm. It promotes proliferation, migration/invasion and metastasis by acting as a miR-548 sponge to increase KIF2C expression, and high circRGNEF levels are associated with aggressive clinicopathological features and poorer overall survival. |
| Confidence score: |
0.8156 |