circRNA basic information
circBase ID: -
Name: hsa_circ_ITCH
Synonym: circ-ITCH
Host Gene: ITCH
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005494
MONDO name: triple-negative breast carcinoma
Disease details: triple-negative breast cancer / TNBC
Disease DO ID:
 0060081
Disease MeSH ID:
 D064726
Disease NCIt ID:
 C71732
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

TNBC tissues; adjacent normal tissues; breast cancer tissues; tumors; lungs
Cell lines:

MCF-10A; MCF-7; T47D; SK-BR-3; MDA-MB-231; BT-549
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: ITCH linear isoform, ITCH, Wnt/beta-catenin signaling, beta-catenin, Axin2, c-Myc, Cyclin D1
Associated microRNA: miR-214, miR-17
Biological function: Circ-ITCH acts as a tumor suppressor in TNBC; its over-expression inhibits TNBC proliferation, migration, invasion, tumor growth and metastasis in vitro and in vivo.
Molecular mechanism: Circ-ITCH functions as a ceRNA/miRNA sponge for miR-214 and miR-17, increases ITCH expression, and attenuates Wnt/beta-catenin signaling.
Biological pathway or process:

Wnt/beta-catenin (inhibits); proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Transwell Assay; Luciferase Reporter Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Cohort Study; Survival Analysis
Clinical significance:

Circ-ITCH is closely associated with lymph node metastasis, larger tumor size, advanced TNM stage and TNBC prognosis; low circ-ITCH expression is associated with shorter survival, and circ-ITCH is proposed as a promising prognostic biomarker for TNBC.
Description:

Circ-ITCH is down-regulated in TNBC tissues and cell lines and acts as a tumor suppressor. It inhibits TNBC proliferation, migration, invasion, growth and metastasis by sponging miR-214 and miR-17, increasing ITCH expression and inactivating Wnt/beta-catenin signaling. Clinically, low circ-ITCH expression is associated with lymph node metastasis, larger tumor size, advanced TNM stage and shorter survival, supporting its value as a prognostic biomarker.
Confidence score:

0.734
Other information
Title:

Circ-ITCH regulates triple-negative breast cancer progression through the Wnt/beta-catenin pathway.

Journal: Neoplasma
Published: 2019
PubMed ID: 30509108
Study type:

combined biological and clinical study
Data availability: -
Code availability: -