| Expression pattern: |
UP |
| Associated gene: |
ROCK1 |
| Associated microRNA: |
miR-532-3p |
| Biological function: |
promotes high glucose-induced HK-2 cell injury (oxidative stress, apoptosis, fibrosis) and inhibits proliferation |
| Molecular mechanism: |
ceRNA mechanism: circ_0000064 sponges miR-532-3p, relieving repression of ROCK1 |
| Biological pathway or process: |
ceRNA regulation (other); apoptosis (promotes); proliferation (inhibits); fibrosis (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
circ_0000064 was increased in serum of DN patients and was higher in macroalbuminuria than microalbuminuria, suggesting association with DN progression |
| Description: |
circ_0000064 is up-regulated in DN patient serum and in HG-treated HK-2 cells, and it aggravates HG-induced renal tubular epithelial cell injury (oxidative stress, apoptosis and fibrosis) while suppressing proliferation. Mechanistically, it functions as a ceRNA by sponging miR-532-3p to increase ROCK1, and knockdown of circ_0000064 alleviates injury via the miR-532-3p/ROCK1 axis. |
| Confidence score: |
0.7045 |