| Expression pattern: |
UP |
| Associated gene: |
CUL4B |
| Associated microRNA: |
miR-335-5p |
| Biological function: |
Circ_0074027 promotes NSCLC cell viability, cell cycle progression, colony formation, and in vivo tumor growth, and inhibits apoptosis. |
| Molecular mechanism: |
Circ_0074027 acts as a miR-335-5p sponge to positively regulate CUL4B expression, thereby promoting NSCLC progression. |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Transfection; CCK8; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Colony Formation Assay; Western Blot; Luciferase Reporter Assay; RNA Pull-Down; In Vivo Animal Model |
| Clinical significance: |
Circ_0074027 level was associated with TNM stages, and higher circ_0074027 level was detected in tissues at worse TNM stage. |
| Description: |
Circ_0074027 is up-regulated in NSCLC tissues and cells and acts as an oncogenic circRNA. Its knockdown inhibits proliferation, colony formation, cell-cycle progression, and xenograft tumor growth while promoting apoptosis. Mechanistically, circ_0074027 sponges miR-335-5p to up-regulate CUL4B expression. |
| Confidence score: |
0.7595 |