circRNA basic information
circBase ID: hsa_circ_0006404
Name: hsa_circ_FOXO3
Synonym: CircFOXO3
Host Gene: FOXO3
Genomic location(hg19): chr6:108984657-108986092:+
Genomic location(hg38): chr6:108663454-108664889:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0018177
MONDO name: glioblastoma
Disease details: glioblastoma / GBM
Disease DO ID:
 3068
Disease MeSH ID:
 D005909
Disease NCIt ID:
 C3058
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

GBM tissues; noncancerous tissues; glioma tissues; normal brain tissues
Cell lines:

U87-MG; U251-MG; A172; T98G; HEBs; HEK293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: NFAT5, AGO2
Associated microRNA: miR-138-5p, miR-432-5p
Biological function: Promotes GBM cell proliferation, colony formation, invasion, migration, tumor growth and invasion in vivo.
Molecular mechanism: circFOXO3 acts as a ceRNA sponge for miR-138-5p and miR-432-5p, thereby increasing NFAT5 expression and promoting GBM progression.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes); other pathway/process (promotes)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; FISH / smFISH; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis
Clinical significance:

High circFOXO3 expression was significantly associated with tumor size, histologic grade, wild-type IDH expression, and MGMT methylation status; circFOXO3 was proposed as a potential therapeutic target in GBM.
Description:

circFOXO3 (hsa_circ_0006404) is up-regulated in GBM tissues and GBM cell lines. It promotes GBM proliferation, invasion, migration, and xenograft tumor growth by acting as a cytoplasmic ceRNA that sponges miR-138-5p and miR-432-5p to increase NFAT5 expression. High circFOXO3 expression is associated with tumor size, histologic grade, IDH status, and MGMT methylation status, supporting its potential as a therapeutic target.
Confidence score:

0.8689
Other information
Title:

CircFOXO3 promotes glioblastoma progression by acting as a competing endogenous RNA for NFAT5.

Journal: Neuro-oncology
Published: 2019
PubMed ID: 31504797
Study type:

combined biological and clinical study
Data availability: Supplementary data are available at Neuro-Oncology online.
Code availability: -