| Expression pattern: |
DN |
| Associated gene: |
TCF21, KLHL20, Cyclin D1, Ki67 |
| Associated microRNA: |
miR-1228 |
| Biological function: |
hsa_circ_100395 acts as a tumor suppressor in lung cancer; its overexpression inhibits lung cancer cell proliferation, arrests cell-cycle progression, reduces migration and invasion, and suppresses tumor growth in vivo. |
| Molecular mechanism: |
hsa_circ_100395 sponges miR-1228 and promotes TCF21 expression, thereby regulating lung cancer cell proliferation, migration and invasion through the miR-1228/TCF21 pathway. |
| Biological pathway or process: |
proliferation (inhibits); cell cycle (other); migration (inhibits); invasion (inhibits); metastasis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis; Transfection; MTT; Cell Cycle Assay; Transwell Assay; RNA Pull-Down; Luciferase Reporter Assay; FISH / smFISH; Western Blot; In Vivo Animal Model |
| Clinical significance: |
hsa_circ_100395 level was inversely correlated with TNM stage and metastases in lung cancer; low hsa_circ_100395 expression predicted poor prognosis, and higher expression predicted better prognosis. |
| Description: |
hsa_circ_100395 is downregulated in lung cancer tissues and functions as a tumor suppressor. It inhibits proliferation, cell-cycle progression, migration, invasion, and xenograft tumor growth by sponging miR-1228 and regulating TCF21. Clinically, lower hsa_circ_100395 expression is associated with higher TNM stage, metastasis, and poorer prognosis. |
| Confidence score: |
0.7855 |