| Expression pattern: |
UP |
| Associated gene: |
HIF1alpha |
| Associated microRNA: |
- |
| Biological function: |
Promotes proliferation of breast cancer cells in a hypoxic environment; associated with tumor progression and larger tumor size. |
| Molecular mechanism: |
HIF1alpha-associated regulation under hypoxia; circDENND4C expression increases after hypoxia induction and decreases after HIF1alpha knockdown, mediating breast cancer cell proliferation in hypoxic conditions. |
| Biological pathway or process: |
proliferation (promotes); HIF-1 signaling (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circDENND4C is highly expressed in breast cancer tissues, positively correlated with HIF1alpha mRNA level, associated with larger tumor size, and may be a substitutive biomarker for HIF1alpha in predicting the clinical impact of breast cancer. |
| Description: |
circDENND4C is an HIF1alpha-associated circRNA up-regulated in breast cancer tissues and in breast cancer cells under hypoxia. Its knockdown suppresses proliferation of MDA-MB-231 cells specifically in hypoxic conditions, and its expression is positively correlated with HIF1alpha mRNA and larger tumor size, suggesting potential clinical biomarker value. |
| Confidence score: |
0.5546 |