| Expression pattern: |
DN |
| Associated gene: |
- |
| Associated microRNA: |
miR-125b |
| Biological function: |
circARF3 overexpression mitigated TNF-alpha-induced inflammatory damage, relieved suppression of cell viability, reduced apoptosis, reduced inflammatory cytokine production, and reduced ROS generation. |
| Molecular mechanism: |
circARF3 positively regulated miR-125b and mitigated TNF-alpha-triggered cell inflammatory damage through inactivation of JNK and NF-kappaB pathways. |
| Biological pathway or process: |
NF-kappaB (inhibits); MAPK (inhibits); apoptosis (inhibits); inflammation (inhibits); other pathway/process (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Transfection; CCK8; Annexin V/PI Flow Cytometry; ELISA; Western Blot; Luciferase Reporter Assay; Bioinformatics Analysis |
| Clinical significance: |
This article might offer a new strategy for the clinical treatment of encephalitis. |
| Description: |
circARF3 was down-regulated in TNF-alpha-induced SH-SY5Y inflammatory damage. Overexpression of circARF3 protected cells by improving viability, reducing apoptosis, cytokine production and ROS generation, and suppressing JNK and NF-kappaB pathway activation through positive regulation of miR-125b. |
| Confidence score: |
0.5607 |