circRNA basic information
circBase ID: hsa_circ_0014130
Name: hsa_circ_PIP5K1A
Synonym: circPIP5K1A
Host Gene: PIP5K1A
Genomic location(hg19): chr1:151206672-151212515:+
Genomic location(hg38): chr1:151234196-151240039:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues; lung tumors
Cell lines:

H1299; PC9; H1975; A549; H1650; BEAS-2B; HEK293T; 293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: HIF-1alpha, Snail, vimentin, E-cadherin
Associated microRNA: miR-600
Biological function: Promotes NSCLC cell proliferation, invasion, migration, tumor growth, and pulmonary metastasis; circPIP5K1A knockdown suppresses these malignant phenotypes.
Molecular mechanism: circPIP5K1A functions as a miR-600 sponge, relieving miR-600-mediated repression of HIF-1alpha and promoting EMT-related protein changes.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); HIF-1 signaling (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; FISH / smFISH; Luciferase Reporter Assay; Transfection; MTT; CCK8; Wound Healing Assay; Transwell Assay; Western Blot; In Vivo Animal Model; Bioinformatics Analysis
Clinical significance:

Potential candidate marker, therapeutic target, and prognostic marker for NSCLC.
Description:

circPIP5K1A (hsa_circ_0014130) is up-regulated in NSCLC cell lines and acts as an oncogenic circRNA. It promotes proliferation, migration, invasion, tumor growth, and pulmonary metastasis by sponging miR-600 and thereby increasing HIF-1alpha expression and EMT-related changes. Silencing circPIP5K1A suppresses NSCLC progression in vitro and in A549 xenograft models.
Confidence score:

0.6004
Other information
Title:

Circular RNA circPIP5K1A promotes non-small cell lung cancer proliferation and metastasis through miR-600/HIF-1alpha regulation.

Journal: Journal of cellular biochemistry
Published: 2019
PubMed ID: 31241217
Study type:

biological research
Data availability: -
Code availability: -