| Expression pattern: |
UP |
| Associated gene: |
MDH1 |
| Associated microRNA: |
hsa-miR-513b-5p |
| Biological function: |
promotes proliferation, migration, and invasion of NSCLC cells; promotes subcutaneous tumor growth in nude mice |
| Molecular mechanism: |
ceRNA mechanism: hsa_circ_0018909 sponges hsa-miR-513b-5p, negatively regulates its expression, and indirectly upregulates MDH1 |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNA-seq; RT-qPCR; Sanger Sequencing; FISH / smFISH; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; In Vivo Animal Model; Clinical Sample Validation; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High hsa_circ_0018909 expression was associated with shorter OS and DFS in NSCLC patients |
| Description: |
hsa_circ_0018909 is upregulated in NSCLC tissues and cell lines and promotes proliferation, migration, invasion, and xenograft tumor growth. It functions mainly in the cytoplasm as a ceRNA that sponges hsa-miR-513b-5p, leading to increased MDH1 expression and poorer patient survival when highly expressed. |
| Confidence score: |
0.8152 |