circRNA basic information
circBase ID: hsa_circ_0001141
Name: hsa_circ_ITCH
Synonym: CircITCH / circ-0001141
Host Gene: ITCH
Genomic location(hg19): chr20:33001547-33037285:+
Genomic location(hg38): chr20:34413741-34449480:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues
Cell lines:

LO2; Huh7; HCCLM3; SMMC-7721; MHCC97H; HepG2
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: -
Associated microRNA: miR-184
Biological function: Inhibits proliferation, migration and invasion; promotes apoptosis; suppresses tumor growth/tumorigenesis in vivo.
Molecular mechanism: Acts as a ceRNA/miRNA sponge binding miR-184; miR-184 rescue reverses circITCH-mediated suppression.
Biological pathway or process:

ceRNA regulation (inhibits); proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes); other pathway/process (other)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Sanger Sequencing; FISH / smFISH; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Transwell Assay; Wound Healing Assay; Annexin V/PI Flow Cytometry; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry)
Clinical significance:

circITCH may serve as a potential prognostic marker and therapeutic target for HCC; a high level of circITCH was associated with favorable survival of HCC.
Description:

circITCH (hsa_circ_0001141), derived from ITCH, is down-regulated in HCC cells and mainly localized in the cytoplasm. It suppresses HCC malignant phenotypes (proliferation, migration, invasion) and promotes apoptosis, partly by sponging miR-184; miR-184 rescue attenuates circITCH’s tumor-suppressive effects. In a cell line-derived xenograft model, circITCH overexpression inhibits tumor growth.
Confidence score:

0.7637
Other information
Title:

Circular RNA CircITCH (has-circ-0001141) suppresses hepatocellular carcinoma (HCC) progression by sponging miR-184.

Journal: Cell cycle (Georgetown, Tex.)
Published: 2022
PubMed ID: 35400275
Study type:

biological research
Data availability: -
Code availability: -