circRNA basic information
circBase ID: hsa_circ_0045602
Name: hsa_circ_HN1
Synonym: circHN1
Host Gene: HN1
Genomic location(hg19): chr17:73142760-73144766:-
Genomic location(hg38): chr17:75146665-75148671:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

gastric tumor tissues; adjacent non-tumor tissues
Cell lines:

HGC-27; MGC-803; GES-1; 293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: AGO2
Associated microRNA: miR-1248, miR-375
Biological function: inhibits gastric cancer cell proliferation, colony formation, migration, and invasion; suppresses tumor growth in vivo
Molecular mechanism: acts as a miRNA sponge and binds to AGO2; sponges miR-1248 and miR-375
Biological pathway or process:

proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (other); ceRNA regulation (other)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; In Vivo Animal Model
Clinical significance:

circHN1 expression was correlated with tumor invasion
Description:

circHN1 (hsa_circ_0045602), derived from the HN1 gene, is up-regulated in gastric cancer tissues and cell lines. Functionally, circHN1 suppresses GC cell proliferation, migration, and invasion in vitro and inhibits xenograft tumor growth in vivo. Mechanistically, circHN1 is mainly cytoplasmic, binds AGO2, and acts as a miRNA sponge for miR-1248 and miR-375.
Confidence score:

0.822
Other information
Title:

CircHN1 affects cell proliferation and migration in gastric cancer.

Journal: Journal of clinical laboratory analysis
Published: 2020
PubMed ID: 32608539
Study type:

combined biological and clinical study
Data availability: GSE83521; GSE93415
Code availability: -