| Expression pattern: |
DN |
| Associated gene: |
PTEN, PI3K/AKT signaling pathway |
| Associated microRNA: |
miR-624-3p |
| Biological function: |
circVRK1 acts as a tumor suppressor in ESCC; overexpression suppresses cell proliferation, migration and EMT, enhances radiosensitivity, and reverses radioresistance. |
| Molecular mechanism: |
circVRK1 functions as a ceRNA/molecular sponge for miR-624-3p, positively regulates PTEN, and inactivates the PI3K/AKT signaling pathway. |
| Biological pathway or process: |
PI3K/AKT (inhibits); proliferation (inhibits); migration (inhibits); EMT (inhibits); radioresistance (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; MTT; Colony Formation Assay; Transwell Assay; Western Blot; IF (Immunofluorescence); Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Bioinformatics Analysis; Survival Analysis |
| Clinical significance: |
High circVRK1 expression is associated with higher overall survival rate, while downregulation of circVRK1 predicts poor prognosis in ESCC patients; circVRK1 may be a novel biomarker and therapeutic target for ESCC. |
| Description: |
circVRK1 is downregulated in ESCC tissues and cell lines, and higher circVRK1 expression is associated with better overall survival. Functionally, circVRK1 suppresses ESCC cell proliferation, migration, EMT and radioresistance. Mechanistically, circVRK1 sponges miR-624-3p to upregulate PTEN and inhibit PI3K/AKT signaling. |
| Confidence score: |
0.7759 |