circRNA basic information
circBase ID: -
Name: hsa_circ_ATAD1
Synonym: hsa_circRNA_100641 / circ-ATAD1
Host Gene: ATAD1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer / GC
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

GC tissue samples; matched normal specimens; cancerous tissues; nontumorous samples
Cell lines:

BGC823; MGC803; GES1; 293T
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: YY1, PCIF1, Ago2
Associated microRNA: miR-140-3p
Biological function: Circ-ATAD1 facilitates GC cell growth, proliferation, colony formation, migration and invasion, and inhibits apoptosis.
Molecular mechanism: Circ-ATAD1 acts through a ceRNA mechanism by sponging miR-140-3p to upregulate YY1; YY1 then directly binds the PCIF1 promoter and activates PCIF1 transcription.
Biological pathway or process:

ceRNA regulation (other); proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); metastasis (promotes)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; Actinomycin D / DRB Stability Assay; Clinical Sample Validation; Cohort Study; Survival Analysis; Bioinformatics Analysis; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; ChIP / ChIP-seq; Western Blot
Clinical significance:

High circ-ATAD1 expression is associated with larger tumor size, deeper invasion, positive lymph node metastasis, advanced TNM stage, adverse prognosis, and is an independent prognostic factor in GC patients.
Description:

Circ-ATAD1 is upregulated in gastric cancer tissues and cell lines and high expression predicts unfavorable prognosis. Functionally, it promotes GC cell proliferation, migration and invasion while inhibiting apoptosis. Mechanistically, circ-ATAD1 acts as a cytoplasmic ceRNA that sponges miR-140-3p to increase YY1, which activates PCIF1 transcription.
Confidence score:

0.8607
Other information
Title:

A novel circular RNA, circ-ATAD1, contributes to gastric cancer cell progression by targeting miR-140-3p/YY1/PCIF1 signaling axis.

Journal: Biochemical and biophysical research communications
Published: 2020
PubMed ID: 32169278
Study type:

combined biological and clinical study
Data availability: TCGA database; circular RNA Interactome; starbase 2.0; TargetScan
Code availability: -