| Expression pattern: |
UP |
| Associated gene: |
PAK1, RUNX1, FUS, ELAVL1, AGO2 |
| Associated microRNA: |
miR-6788-5p |
| Biological function: |
promotes proliferation, migration, and invasion; inhibits autophagy; promotes tumor growth in vivo |
| Molecular mechanism: |
ceRNA sponging of miR-6788-5p to regulate PAK1, activating Akt and Erk phosphorylation and modulating autophagy; biogenesis regulated by RUNX1 (negative) and RBPs FUS/ELAVL1 (positive); feedback loop between autophagy inhibition and FUS-mediated circPTPN22 upregulation |
| Biological pathway or process: |
autophagy (inhibits); proliferation (promotes); migration (promotes); invasion (promotes); PI3K/AKT (promotes); ERK (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; Western Blot; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; ChIP / ChIP-seq; In Vivo Animal Model; IHC (Immunohistochemistry); IF (Immunofluorescence); Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
circPTPN22 can serve as a biomarker for GC and can be a potential therapeutic target. |
| Description: |
In gastric cancer, circPTPN22 is up-regulated and promotes proliferation, migration, invasion, and in vivo tumor growth while inhibiting autophagy. It mainly functions as a ceRNA by sponging miR-6788-5p to upregulate PAK1, activating Akt and Erk phosphorylation to suppress autophagy. circPTPN22 biogenesis is negatively regulated by RUNX1 and positively regulated by RBPs FUS and ELAVL1, and autophagy inhibition forms a positive feedback loop via increased FUS that further elevates circPTPN22. |
| Confidence score: |
0.8109 |