| Expression pattern: |
UP |
| Associated gene: |
FoxO3, Bim EL, Cytochrome c, Cleaved caspase-3, Caspase-3 |
| Associated microRNA: |
- |
| Biological function: |
Endogenous circFoxO3 promotes glutamate-induced oxidative injury, ROS accumulation, mitochondrial membrane potential loss, mitochondrial apoptosis, and neuronal cell death in HT22 cells; silencing circFoxO3 protects HT22 cells from these injuries. |
| Molecular mechanism: |
circFoxO3 contributes to mitochondrial-mediated apoptosis by activating the FoxO3/Bim EL pathway and regulating Cytochrome c/Caspase-3 mitochondrial-related apoptotic signalling. |
| Biological pathway or process: |
apoptosis (promotes); mitochondrial function (inhibits); other pathway/process (promotes) |
| Detected method: |
Q
H
|
| Validation methods: |
Sanger Sequencing; RT-qPCR; FISH / smFISH; IF (Immunofluorescence); Transfection; TUNEL; Western Blot |
| Clinical significance: |
circFoxO3 may act as a novel therapeutic target and potential diagnostic marker for neurodegenerative diseases. |
| Description: |
This study found that circFoxO3 is up-regulated in glutamate-treated mouse HT22 neuronal cells, a model of neurodegenerative oxidative injury. circFoxO3 promotes oxidative stress-related mitochondrial apoptosis through the FoxO3/Bim EL and Cytochrome c/Caspase-3 mitochondrial apoptotic pathway, while circFoxO3 knockdown reduces ROS accumulation, MMP loss, apoptosis, and cell death. The authors suggest circFoxO3 as a potential diagnostic marker and therapeutic target for neurodegenerative diseases. |
| Confidence score: |
0.5627 |