circRNA basic information
circBase ID: -
Name: hsa_circ_RANBP17
Synonym: circRANBP17
Host Gene: RAN binding protein 17
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005072
MONDO name: neuroblastoma
Disease details: neuroblastoma / NB
Disease DO ID:
 769
Disease MeSH ID:
 D009447
Disease NCIt ID:
 C3270
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NB tissues; adjacent normal tissues; normal brain tissues; primary tumors (xenograft)
Cell lines:

SK-N-SH; IMR-32; SK-N-AS; BE(2)-C; HUVEC
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: KDM1A, Ago2
Associated microRNA: miR-27b-3p
Biological function: promotes proliferation, migration and invasion; inhibits apoptosis; promotes tumor formation in vivo
Molecular mechanism: Acts as a miRNA sponge for miR-27b-3p to de-repress KDM1A (ceRNA-like regulation); associated with Ago2 complex
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); metastasis (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Nuclear-Cytoplasmic Fractionation; Actinomycin D / DRB Stability Assay; Dual-luciferase reporter assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; MTT; EdU Staining; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation
Clinical significance:

-
Description:

circRANBP17 is up-regulated in neuroblastoma tissues/cells and mainly localized in the cytoplasm. It promotes NB malignant behaviors (proliferation, migration, invasion, tumor growth) and inhibits apoptosis by sponging miR-27b-3p to increase KDM1A expression.
Confidence score:

0.8201
Other information
Title:

CircRANBP17 modulated KDM1A to regulate neuroblastoma progression by sponging miR-27b-3p.

Journal: Open medicine (Warsaw, Poland)
Published: 2023
PubMed ID: 36941992
Study type:

combined biological and clinical study
Data availability: The original western blots pictures and datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
Code availability: -