| Expression pattern: |
UP |
| Associated gene: |
PARP1, eIF4A3, AGO2 |
| Associated microRNA: |
- |
| Biological function: |
Promotes prostate cancer cell proliferation, migration/invasion and metastasis; inhibits apoptosis; relieves DNA damage; exosome-delivered circTFDP2 promotes PCa progression. |
| Molecular mechanism: |
circTFDP2 binds PARP1 (DNA-binding domain) and prevents active caspase-3-dependent cleavage of PARP1, thereby relieving DNA damage; eIF4A3 binds flanking region and promotes circTFDP2 biogenesis; circTFDP2 is enriched in exosomes and mediates pro-tumor effects. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Sanger Sequencing; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; IF (Immunofluorescence); RNA Pull-Down; RIP (RNA Immunoprecipitation); Bioinformatics Analysis; In Vivo Animal Model |
| Clinical significance: |
high circTFDP2 expression was positively correlated with the Gleason score |
| Description: |
circTFDP2 (hsa_circ_0008304) is upregulated in prostate cancer and correlates with more aggressive clinicopathologic features. It promotes PCa proliferation and metastasis and reduces apoptosis and DNA damage by binding PARP1 and preventing caspase-3-dependent PARP1 cleavage. circTFDP2 is enriched in exosomes from PCa cells and exosome-delivered circTFDP2 further enhances PCa progression. |
| Confidence score: |
0.8446 |