| Expression pattern: |
UP |
| Associated gene: |
FMNL2 |
| Associated microRNA: |
miR-1207-5p |
| Biological function: |
circHIPK3 serves as an oncogene and promotes CRC cell proliferation, migration, invasion and metastasis; knockdown suppresses migration, invasion and proliferation. |
| Molecular mechanism: |
circHIPK3 acts as a cytoplasmic ceRNA/miRNA sponge for miR-1207-5p, thereby upregulating FMNL2 protein and promoting CRC cell proliferation, migration and invasion. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
circHIPK3 is closely related with unfavorable clinicopathological features and higher expression is correlated with shorter survival in CRC patients; circHIPK3/miR-1207-5p/FMNL2 axis might be a novel therapeutic target. |
| Description: |
circHIPK3 is up-regulated in colorectal cancer tissues and cell lines, and high expression is associated with unfavorable clinicopathological features and poorer survival. Functionally, circHIPK3 promotes CRC cell proliferation, migration and invasion by sponging miR-1207-5p and enhancing FMNL2 protein expression, suggesting the circHIPK3/miR-1207-5p/FMNL2 axis as a potential therapeutic target. |
| Confidence score: |
0.8323 |