| Expression pattern: |
UP |
| Associated gene: |
E2F2, Ago2 |
| Associated microRNA: |
hsa-miR-432-5p, hsa-miR-326 |
| Biological function: |
Promotes proliferation, migration, invasion, tube formation, EMT, tumorigenesis and metastasis in NSCLC; its knockdown inhibits these malignant phenotypes. |
| Molecular mechanism: |
circPTN acts as a miRNA sponge for miR-432-5p, thereby upregulating E2F2 (ceRNA-like regulation) and promoting malignant phenotypes of NSCLC cells. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); metastasis (promotes); angiogenesis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Survival Analysis; Transfection; CCK8; EdU Staining; Transwell Assay; Tube Formation Assay; Western Blot; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; Bioinformatics Analysis |
| Clinical significance: |
High circPTN expression is associated with lymph node metastasis and advanced TNM stage, and can serve as a prognostic marker; high expression correlates with worse overall survival in NSCLC patients. |
| Description: |
circPTN is up-regulated in NSCLC tissues/cells and promotes malignant behaviors (proliferation, migration/invasion, EMT and angiogenesis-like tube formation). Mechanistically, it sponges miR-432-5p to upregulate E2F2, and high circPTN expression is associated with worse overall survival, suggesting prognostic value. |
| Confidence score: |
0.7976 |