circRNA basic information
circBase ID: -
Name: hsa_circ_HIAT1
Synonym: circHIAT1 / CircRNA Hippocampus Abundant Transcript 1
Host Gene: HIAT1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer / GC
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

GC tissues; related nontumor tissues; nontumor adjacent tissues
Cell lines:

MKN28; MKN45
In vivo animal model:

-
circRNA-disease information
Expression pattern:
DN
Associated gene: PTEN, PI3K, AKT, ERK, p53, p21, E-cadherin, N-cadherin, vimentin, ZEB1, cleaved caspase-3, cleaved caspase-8, cleaved caspase-9
Associated microRNA: miR-21
Biological function: circHIAT1 functions as a tumor inhibitor in gastric cancer cells; its overexpression suppresses cell proliferation and EMT, induces apoptosis, decreases cell viability, and inhibits PTEN/PI3K/AKT and ERK pathway activation.
Molecular mechanism: circHIAT1 negatively regulates miR-21, thereby increasing PTEN and suppressing PI3K/AKT and ERK signaling; miR-21 mimic reverses the growth-inhibitory, pro-apoptotic and EMT-suppressive effects of circHIAT1 overexpression.
Biological pathway or process:

proliferation (inhibits); apoptosis (promotes); EMT (inhibits); PI3K/AKT (inhibits); ERK (inhibits); p53 signaling (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Annexin V/PI Flow Cytometry; Western Blot
Clinical significance:

circHIAT1 could be a novel target for GC treatment and a promising effective therapeutic target in GC.
Description:

In this study, circHIAT1 was down-regulated in gastric cancer tissues and acted as a tumor suppressor in MKN28 and MKN45 gastric cancer cells. circHIAT1 overexpression inhibited proliferation and EMT, promoted apoptosis, and suppressed PTEN/PI3K/AKT and ERK signaling by downregulating miR-21, suggesting therapeutic potential in gastric cancer.
Confidence score:

0.5358
Other information
Title:

Circular RNA circHIAT1 inhibits proliferation and epithelial-mesenchymal transition of gastric cancer cell lines through downregulation of miR-21.

Journal: Journal of biochemical and molecular toxicology
Published: 2020
PubMed ID: 32020707
Study type:

combined biological and clinical study
Data availability: -
Code availability: -