circRNA basic information
circBase ID: hsa_circ_0005526
Name: hsa_circ_RUNX2
Synonym: circ_0005526
Host Gene: RUNX2
Genomic location(hg19): chr6:45459677-45460699:+
Genomic location(hg38): chr6:45491940-45492962:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005178
MONDO name: osteoarthritis
Disease details: osteoarthritis / OA
Disease DO ID:
 8398
Disease MeSH ID:
 D010003
Disease NCIt ID:
 C3293
Disease ICD11 ID:
 558562409
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

OA cartilage tissues; normal cartilage tissues
Cell lines:

CHON-001
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: TCF4
Associated microRNA: miR-142-5p
Biological function: Promotes IL-1beta-induced chondrocyte injury by decreasing viability and proliferation and increasing apoptosis and inflammation in an OA cell model.
Molecular mechanism: Acts as a ceRNA/miRNA sponge for miR-142-5p, thereby relieving miR-142-5p-mediated repression of TCF4 and increasing TCF4 expression.
Biological pathway or process:

proliferation (inhibits); apoptosis (promotes); inflammation (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; RNA Pull-Down; Clinical Sample Validation; Bioinformatics Analysis
Clinical significance:

-
Description:

circ_0005526 is up-regulated in OA cartilage and IL-1beta-treated chondrocytes. It promotes IL-1beta-induced chondrocyte injury by sponging miR-142-5p, thereby increasing TCF4 expression and enhancing apoptosis and inflammatory responses while reducing viability/proliferation.
Confidence score:

0.7581
Other information
Title:

Circ_0005526 contributes to interleukin-1beta-induced chondrocyte injury in osteoarthritis via upregulating transcription factor 4 by interacting with miR-142-5p.

Journal: Bioengineered
Published: 2022
PubMed ID: 35322736
Study type:

combined biological and clinical study
Data availability: Supplemental data for this article can be accessed here.
Code availability: -