| Expression pattern: |
UP |
| Associated gene: |
UBE2A, MDM2, p53, Bcl2, Bax |
| Associated microRNA: |
miR-527 |
| Biological function: |
promotes proliferation, migration, and invasion; promotes tumorigenesis in vivo |
| Molecular mechanism: |
Acts as a sponge of miR-527 to regulate UBE2A, involving the MDM2/p53/Bcl2/Bax pathway and EMT-related markers. |
| Biological pathway or process: |
ceRNA regulation (promotes); EMT (promotes); apoptosis (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RNase R Treatment; Sanger Sequencing; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; EdU Staining; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Western Blot; IHC (Immunohistochemistry); H&E Staining; In Vivo Animal Model; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High expression of hsa_circ_0001394 was related to poor survival and correlated with tumor differentiation, vascular invasion, and AFP levels; increased plasma expression correlated with clinicopathological features including elevated vascular invasion and an advanced TNM stage. |
| Description: |
hsa_circ_0001394 (circTBC1D14) is up-regulated in HCC tissues/cells/plasma and mainly localized in the cytoplasm. It promotes HCC cell proliferation, migration, invasion and tumor growth by sponging miR-527, thereby relieving repression of UBE2A and affecting downstream MDM2/p53/Bcl2/Bax and EMT-related changes. |
| Confidence score: |
0.8658 |