| Expression pattern: |
UP |
| Associated gene: |
RAP2C |
| Associated microRNA: |
miR-136-5p |
| Biological function: |
Promotes LSCC cell proliferation, colony formation, migration, invasion and in vivo tumor growth, while inhibiting apoptosis; high expression correlates with advanced TNM stage and lymph node metastasis. |
| Molecular mechanism: |
circRNA_100290 acts as a miR-136-5p sponge to relieve miR-136-5p-mediated repression of the oncogene RAP2C, thereby promoting LSCC progression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); metastasis (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Western Blot; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model |
| Clinical significance: |
High circRNA_100290 expression positively correlated with advanced TNM stage and lymph node metastasis in LSCC patients, suggesting potential therapeutic relevance. |
| Description: |
circRNA_100290 is upregulated in LSCC tissues and cell lines and is associated with advanced TNM stage and lymph node metastasis. It promotes malignant LSCC phenotypes including proliferation, colony formation, migration, invasion and xenograft tumor growth, while suppressing apoptosis. Mechanistically, it functions as a ceRNA for miR-136-5p to upregulate RAP2C. |
| Confidence score: |
0.6544 |