| Expression pattern: |
UP |
| Associated gene: |
RAC1, RSF1 |
| Associated microRNA: |
miR-146a-5p |
| Biological function: |
Promotes cell viability, proinflammatory cytokine production, and fibrotic phenotype/cell activation in irradiated hepatic stellate cells. |
| Molecular mechanism: |
circRSF1 acts as a miR-146a-5p sponge, counteracts miR-146a-5p-mediated repression of RAC1, increases RAC1 expression/activity, and activates RAC1 downstream NF-kappaB and JNK/Smad2 signaling. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); inflammation (promotes); fibrosis (promotes); NF-kappaB (promotes); MAPK (promotes); TGF-beta/SMAD (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Microarray; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; ELISA; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circRSF1 may be a potential therapeutic target for prevention and treatment of RILD. |
| Description: |
circRSF1 is up-regulated in irradiated human hepatic stellate LX2 cells, a cellular model relevant to radiation-induced liver disease. It promotes inflammatory cytokine production, cell viability, and fibrotic activation by sponging miR-146a-5p, thereby relieving repression of RAC1 and activating RAC1-associated NF-kappaB and JNK/Smad2 signaling. The study suggests circRSF1 as a potential therapeutic target for RILD. |
| Confidence score: |
0.7296 |