| Expression pattern: |
UP |
| Associated gene: |
Ago2, p-AKT, VEGF, b-catenin |
| Associated microRNA: |
miR-184 |
| Biological function: |
cZNF609 promotes corneal neovascularization, HCEK cell proliferation and migration, and endothelial tube formation; its overexpression rescues the inhibitory effects of miR-184. |
| Molecular mechanism: |
cZNF609 acts as a miR-184 sponge and regulates downstream AKT/b-catenin/VEGF signaling. |
| Biological pathway or process: |
PI3K/AKT (promotes); VEGF/VEGFR (promotes); Wnt/beta-catenin (promotes); proliferation (promotes); migration (promotes); angiogenesis (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Bioinformatics Analysis; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; Wound Healing Assay; Tube Formation Assay; Western Blot; In Vivo Animal Model |
| Clinical significance: |
Targeting cZNF609 may serve as a promising therapeutic method for pathological corneal neovascularization. |
| Description: |
cZNF609 is up-regulated during corneal neovascularization and promotes pathological angiogenesis. Mechanistically, it sponges miR-184, relieving miR-184-mediated inhibition of AKT/b-catenin/VEGF signaling and thereby enhancing cell proliferation, migration, and tube formation. Targeting cZNF609 is proposed as a potential therapeutic strategy for pathological corneal neovascularization. |
| Confidence score: |
0.6578 |