| Expression pattern: |
UN |
| Associated gene: |
- |
| Associated microRNA: |
- |
| Biological function: |
circFBXW7 acts as a tumor suppressor in AML and regulates the proliferative capacity of AML blasts; circFBXW7 knockdown increased proliferation and colony formation. |
| Molecular mechanism: |
The mechanism is unlikely to be miRNA sequestration; high circFBXW7 expression was associated with genes involved in chromatin state, transcription, leukocyte activation and differentiation, whereas low expression was associated with stem cell properties and WNT/beta-catenin and NOTCH signaling. |
| Biological pathway or process: |
proliferation (inhibits); Wnt/beta-catenin (inhibits); Notch (inhibits); stemness (inhibits); other pathway/process (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RNase R Treatment; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Transfection; BrdU; Colony Formation Assay; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
circFBXW7 functions as a tumor suppressor-like circRNA in AML. Its knockdown increases proliferation of AML cell lines and colony formation by patient AML blasts, and its mechanism is unlikely to involve miRNA sequestration based on lack of detectable miRNA-binding sites. |
| Confidence score: |
0.6716 |