| Expression pattern: |
UP |
| Associated gene: |
VGF |
| Associated microRNA: |
miR-451a |
| Biological function: |
promotes peripheral neural remodeling and axon elongation/neurite extension in DRG; promotes neuronal survival; promotes tumor proliferation and increases intra-tumoral nerve density |
| Molecular mechanism: |
exosomal circRNA transferred from PDAC cells to DRG acts as a ceRNA sponge for miR-451a to relieve repression of VGF |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); invasion (promotes); apoptosis (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; Western Blot; CCK8; Transwell Assay; Annexin V/PI Flow Cytometry; In Vivo Animal Model; IHC (Immunohistochemistry); IF (Immunofluorescence); Clinical Sample Validation; Cohort Study |
| Clinical significance: |
plasma exosomal circ-0011536 is associated with PNR and may serve as a prognostic biomarker to predict PNR in pancreatic cancer |
| Description: |
In PDAC with activated Hedgehog/Gli1 signaling, circ-0011536 is enriched in tumor-derived exosomes and transferred to DRG cells, where it sponges miR-451a to increase VGF expression and promote neurite outgrowth/neural remodeling (PNR). Plasma exosomal circ-0011536 is elevated in patients with PNR, supporting its potential as a biomarker for PDAC-associated nerve alterations. |
| Confidence score: |
0.7631 |