| Expression pattern: |
DN |
| Associated gene: |
BTG1, Ago2 |
| Associated microRNA: |
miR-421 |
| Biological function: |
Tumor suppressor; overexpression induces cell cycle arrest and apoptosis, reduces cell viability and colony formation, and restrains HCC development. |
| Molecular mechanism: |
circITCH functions as a ceRNA sponge for miR-421 to relieve miR-421-mediated inhibition of BTG1, thereby elevating BTG1 expression and regulating cell cycle, proliferation and apoptosis. |
| Biological pathway or process: |
ceRNA regulation (other); proliferation (inhibits); apoptosis (promotes); cell cycle (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; MTT; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot |
| Clinical significance: |
Lower circITCH expression was closely associated with larger tumor size and higher TNM stage, suggesting it may be a potential indicator for HCC progression. |
| Description: |
circITCH is down-regulated in HCC tissues and cell lines and acts as a tumor suppressor. It inhibits proliferation and colony formation, induces apoptosis and G1/S cell cycle arrest, and functions through a circITCH/miR-421/BTG1 ceRNA axis. Lower circITCH expression is associated with larger tumor size and higher TNM stage, indicating potential clinical relevance in HCC progression. |
| Confidence score: |
0.7045 |