| Expression pattern: |
UP |
| Associated gene: |
HuR, Atg16l1 mRNA, ATG16L1 |
| Associated microRNA: |
- |
| Biological function: |
circPABPN1 suppresses ATG16L1 expression by preventing HuR association with Atg16l1 mRNA, thereby repressing ATG16L1 translation and contributing to defective autophagy in the intestinal epithelium. |
| Molecular mechanism: |
circPABPN1 interacts with the RNA-binding protein HuR and sequesters HuR away from Atg16l1 mRNA, reducing HuR-mediated ATG16L1 translation without altering Atg16l1 mRNA levels. |
| Biological pathway or process: |
autophagy (inhibits); other pathway/process (inhibits) |
| Detected method: |
H
|
| Validation methods: |
RNase R Treatment; RT-qPCR; FISH / smFISH; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Transfection; Western Blot |
| Clinical significance: |
circPABPN1 is increased in human mucosal tissues from UC patients and this increase is associated with decreased ATG16L1, suggesting relevance to IBD mucosal pathology and a potential therapeutic axis. |
| Description: |
circPABPN1 is up-regulated in intestinal mucosal tissues from UC patients. Mechanistically, circPABPN1 binds/sequesters HuR, prevents HuR association with Atg16l1 mRNA, and reduces ATG16L1 translation, thereby antagonizing HuR-regulated autophagy in intestinal epithelial cells. |
| Confidence score: |
0.6875 |