| Expression pattern: |
UP |
| Associated gene: |
ULK1, Ago2, MFN2 mRNA |
| Associated microRNA: |
miR-34a-5p |
| Biological function: |
promotes imatinib resistance, cell proliferation and autophagy; inhibits imatinib-induced apoptosis in CML cells |
| Molecular mechanism: |
circ_0009910 sponges miR-34a-5p to promote ULK1 expression and ULK1-induced autophagy, thereby facilitating imatinib resistance |
| Biological pathway or process: |
autophagy (promotes); apoptosis (inhibits); proliferation (promotes); drug resistance (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Annexin V/PI Flow Cytometry; Western Blot; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); ROC Analysis; Survival Analysis |
| Clinical significance: |
up-regulated in serum of imatinib-resistant CML patients, associated with unfavorable clinicopathologic features, diagnostic value for imatinib resistance, and high expression predicts shorter overall survival |
| Description: |
circ_0009910 is up-regulated in serum from imatinib-resistant CML patients and in K562/R cells. It promotes imatinib resistance by sponging miR-34a-5p, increasing ULK1 expression and ULK1-induced autophagy, while reducing apoptosis. Clinically, high circ_0009910 expression is associated with diagnostic value for resistance and poorer overall survival. |
| Confidence score: |
0.8569 |